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KMID : 1189120070040020179
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대한의학유전학회지
2007 Volume.4 No. 2 p.179 ~ p.185
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Effect of immobilization stress on the expression of TH, BDH and CRH gene in rat brain
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Qian Yong-Ri
Kim Yoon-Sik
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Abstract
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Purpose : Catecholamines are the neuro-transmitters in the Sympathetic nervous System (SNS) and are activated by stress stimulus. Tyrosine hydroxylase (TH) and Dopamine-β -Hydroxylase (DBH) are very important enzymes in the catecholamine synthesis. Corticotropin releasing hormone (CRH) is released in the process of reacting to stresses. The aim of this study is to find out what effects immobilization stresses have on the expression of TH, BDH and CRH mRNA in a rat’s brains.
Methods : We Compare expression levels in rat’S brains Of TH, DBH and CRH mRNA induced by immobilization stresses between the test group and controled group. The expression levels of TH, DBH and CRH mRNA are measured by RT -PCR and the Western Blotting Analysis (WBA).
Results : In brains and adrenal 91an0s of the immobilization stress group, the expression levels of TH and DBH mRNAs are significantly two to three times higher (P<0.01), and CRH mRNAs are approxi- mately one and a half times higher (P<0.05) than those of controlled group.
Conclusion : This study suggest that the expression levels of TH, DBH and CRH mRNAs are activated by stress stimulus in a rat’s brains and adrenal glands.
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KEYWORD
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Immobilization stress, TH, DBH and CRH mRNAs
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